Curdlan induces DC-mediated Th17 polarization via Jagged1 activation in human dendritic cells.
Allergol Int
; 59(2): 161-6, 2010 Jun.
Article
in En
| MEDLINE
| ID: mdl-20179419
ABSTRACT
BACKGROUND:
Th17-inducing activity is carried by certain polysaccharides such as beta-glucan derived from Candia albicans. Our previous studies have shown that Th1- and Th2-inducing activities can be qualitatively evaluated by the expression patterns of Notch ligand isoforms, using human monocyte-derived dendritic cells (Mo-DCs) and some leukemic cell lines such as THP-1. The association of Th17-inducing activities with Notch ligand expression patterns has been unclear.METHODS:
Mo-DCs from healthy volunteers were co-cultured with HLA-DR-nonshared allogeneic CD4+ naïve T cells to induce a mixed lymphocyte reaction, in the presence of adjuvants, such as curdlan. Culture supernatants were assayed for IFNgamma, IL-5 and IL-17 by an enzyme-linked immunosorbent assay (ELISA). Notch ligand expression on Mo-DCs and THP-1 cells was evaluated by using RT-PCR.RESULTS:
The present study shows that curdlan, one of the beta-glucans, has the ability to induce DC-mediated Th17 differentiation. It is also interesting to note that Jagged1 mRNA in Mo-DCs and THP-1 cells is up-regulated by curdlan. Furthermore, polyclonal anti-Jagged1 antibody inhibited such DC-mediated Th17 differentiation.CONCLUSIONS:
This study suggests that curdlan induces human DC-mediated Th17 polarization via Jagged1 activation in DCs.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides, Bacterial
/
Dendritic Cells
/
Calcium-Binding Proteins
/
Candida albicans
/
CD4-Positive T-Lymphocytes
/
Interleukin-17
/
Intercellular Signaling Peptides and Proteins
/
Beta-Glucans
/
Membrane Proteins
Limits:
Humans
Language:
En
Journal:
Allergol Int
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Japan