Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration.

Lombardi, Raffaele; Villani, Maria Elena; Di Carli, Mariasole; Brunetti, Patrizia; Benvenuto, Eugenio; Donini, Marcello

Lombardi, Raffaele; Villani, Maria Elena; Di Carli, Mariasole; Brunetti, Patrizia; Benvenuto, Eugenio; Donini, Marcello.

Affiliation

Lombardi R; ENEA, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, C.R. Casaccia, Via Anguillarese 301, 00123, Rome, Italy.

Transgenic Res ; 19(6): 1083-97, 2010 Dec.

Article in En | MEDLINE | ID: mdl-20229286

Subject(s)

Antibodies, Neoplasm/genetics; Antibodies, Neoplasm/isolation & purification; Nicotiana/genetics; Nicotiana/immunology; Plantibodies/genetics; Plantibodies/isolation & purification; Agrobacterium tumefaciens/genetics; Antibodies, Neoplasm/biosynthesis; Blotting, Western; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Endotoxins/isolation & purification; Enzyme-Linked Immunosorbent Assay; Gene Expression; Humans; Immunoglobulin G/biosynthesis; Immunoglobulin G/genetics; Immunoglobulin G/isolation & purification; Pilot Projects; Plant Leaves/immunology; Plantibodies/metabolism; Plants, Genetically Modified; Protein Engineering; Surface Plasmon Resonance; Vacuum

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nicotiana / Plantibodies / Antibodies, Neoplasm Type of study: Evaluation_studies Limits: Humans Language: En Journal: Transgenic Res Journal subject: BIOLOGIA MOLECULAR Year: 2010 Document type: Article Affiliation country: Italy Country of publication: Netherlands

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