A histidine substitution confers metal binding affinity to a Schistosoma japonicum Glutathione S-transferase.

Han, Young-Hyun; Seo, Hee-Ah; Kim, Ga-Hye; Lee, Chung-Kyung; Kang, Young Kee; Ryu, Keun Ho; Chung, Yong Je

Han, Young-Hyun; Seo, Hee-Ah; Kim, Ga-Hye; Lee, Chung-Kyung; Kang, Young Kee; Ryu, Keun Ho; Chung, Yong Je.

Affiliation

Han YH; Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea.

Protein Expr Purif ; 73(1): 74-7, 2010 Sep.

Article in En | MEDLINE | ID: mdl-20347989

Subject(s)

Chromatography, Affinity/methods; Glutathione Transferase/metabolism; Histidine/metabolism; Nickel/metabolism; Recombinant Fusion Proteins/metabolism; Schistosoma japonicum/enzymology; Amino Acid Sequence; Amino Acid Substitution; Animals; Biotin/genetics; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Glutathione Transferase/chemistry; Glutathione Transferase/genetics; Histidine/chemistry; Histidine/genetics; Humans; Models, Molecular; Molecular Sequence Data; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Schistosoma japonicum/genetics; Sequence Alignment; Structure-Activity Relationship

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Schistosoma japonicum / Recombinant Fusion Proteins / Chromatography, Affinity / Glutathione Transferase / Histidine / Nickel Limits: Animals / Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2010 Document type: Article Country of publication: United States

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