Polyacrylic microspheres as a solid phase for microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) of transferrin.
J Immunoassay
; 12(2): 165-83, 1991.
Article
in En
| MEDLINE
| ID: mdl-2045477
ABSTRACT
Polyfunctional hydrophilic microspheres (MS) can be produced by copolymerisation with gamma-irradiation of acrylic monomers. Transferrin (TRF) can be covalently bound to these MS by reaction between aldehyde groups of the MS and primary amino groups of the protein. MS-TRF conjugates thus obtained are agglutinated by specific antiserum and this agglutination is inhibited by free TRF. Agglutination and inhibition are quantified by measurement of the light scattered by MS-TRF conjugate clusters with a specially designed nephelometer, a process designated as microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) for TRF. Recovery, correlation and reproducibility studies, simultaneously performed in three different laboratories, show that this TRF immunoassay is accurate for a large concentration range. NEPHELIA (R) may appear as an alternative method for a large variety of molecules.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transferrin
/
Immunoassay
/
Nephelometry and Turbidimetry
Limits:
Humans
Language:
En
Journal:
J Immunoassay
Year:
1991
Document type:
Article
Affiliation country:
France