Human trophoblast responses to Porphyromonas gingivalis infection.
Mol Oral Microbiol
; 25(4): 252-9, 2010 Aug.
Article
in En
| MEDLINE
| ID: mdl-20618699
ABSTRACT
Porphyromonas gingivalis is a periodontal pathogen that is also associated with preterm low-birthweight delivery. We investigated the transcriptional responses of human extravillous trophoblasts (HTR-8) to infection with P. gingivalis. Over 2000 genes were differentially regulated in HTR-8 cells by P. gingivalis. In ontology analyses of regulated genes, overpopulated biological pathways included mitogen-activated protein (MAP) kinase signaling and cytokine production. Immunoblots confirmed overexpression of the MAP kinase pathway components MEK3, p38 and Max. Furthermore, P. gingivalis infection induced phosphorylation and activation of MEK3 and p38. Increased production of interleukin (IL)-1beta and IL-8 by HTR-8 cells was demonstrated phenotypically by enzyme-linked immunosorbent assay of HTR-8 cell lysates and culture supernatants. Hence, infection of trophoblasts by P. gingivalis can impact signal transduction pathways and modulate cytokine expression, outcomes that could disrupt the maintenance of pregnancy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trophoblasts
/
Interleukins
/
Bacteroidaceae Infections
/
Porphyromonas gingivalis
/
Premature Birth
Type of study:
Etiology_studies
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Mol Oral Microbiol
Year:
2010
Document type:
Article
Affiliation country:
United States
Country of publication:
DENMARK
/
DINAMARCA
/
DK