Prolonged cardiac allograft survival using iodine 131 after human sodium iodide symporter gene transfer in a rat model.
Transplant Proc
; 42(5): 1888-94, 2010 Jun.
Article
in En
| MEDLINE
| ID: mdl-20620544
ABSTRACT
BACKGROUND:
Radioiodine is efficiently concentrated by tissues expressing the human sodium iodide symporter (hNIS).OBJECTIVE:
To analyze the effects of iodine 131 on acute cardiac allograft rejection after ex vivo hNIS gene transfer in a rat model of cardiac allotransplantation. MATERIALS ANDMETHODS:
Hearts from Brown Norway rats were perfused ex vivo either with UW (University of Wisconsin) solution (n = 9) or UW solution containing 1 x 10(9) pfu/mL of adenovirus 5 plus NIS (Ad-NIS) (n = 18). Donor hearts were transplanted heterotopically into the abdomen of Lewis rats, and recipients were treated on postoperative day 3 with either 15,000 microCi of (131)I or saline solution. The hearts were explanted when no longer beating, and were evaluated histologically for evidence of rejection and other changes.RESULTS:
Grafts perfused with the Ad-NIS vector survived significantly longer in recipients injected with (131)I (mean [SD], 11.3 [1.9] days) compared with control animals not treated with (131)I (5.7 [0.65] days) (P < .001). Treatment with (131)I did not prolong graft survival in recipients of hearts that were not perfused with Ad-NIS (5.5 [1.0] vs 5.3 [0.8] days). In Ad-NIS (131)I-treated transplants, the level of myocardial damage on day 6 after surgery, when control hearts were rejected, was significantly lower (60.8 [28.0] vs 99.7 [0.8]; P < .05).CONCLUSION:
Our findings indicate that (131)I, after NIS gene transfer, can effectively prolong cardiac allograft survival. To our knowledge, this is the first report of the use of NIS-targeted (131)I therapy in cardiac transplantation. Further studies are required to determine the mechanism of this effect and its potential for clinical application.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transplantation, Homologous
/
Heart Transplantation
/
Symporters
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Transplant Proc
Year:
2010
Document type:
Article
Affiliation country:
United States