Functional analysis of carp interferon-gamma: evolutionary conservation of classical phagocyte activation.
Fish Shellfish Immunol
; 29(5): 793-802, 2010 Nov.
Article
in En
| MEDLINE
| ID: mdl-20633651
ABSTRACT
In teleost fish two IFN-gamma gene sequences were found for which two phylogenetic clusters can be distinguished. Our previous analysis of expression of these in carp led us to hypothesize that a classical IFN-gamma function is associated with the IFN-gamma2 cluster. We investigated the evolutionary conservation of the IFN-gamma function, inducing classical activation of phagocytes, thus skewing towards a Th1-like profile of immune activation. Recombinant proteins for the carp IFN-gamma sequences of both clusters were made and we studied their effects on expression of proinflammatory mediators. Carp IFN-gamma2, in contrast to carp IFN-gamma1, was powerful in inducing a proinflammatory reaction in phagocytes a classical synergistic response with lipopolysaccharide was observed for the induction of iNOS expression and NO release, for expression of CXCL9-11-like chemokines and the expression of proinflammatory cytokines IL-1beta, TNFalpha and the IL-12 subunits p35 and p40. In contrast, like in mammals, the CXCL8-like cytokines are LPS but not IFN-gamma sensitive. These results corroborate an evolutionary conserved nature of IFN-gamma function in lower vertebrates including classical activation of phagocytes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phagocytes
/
Carps
/
Interferon-gamma
/
Biological Evolution
Limits:
Animals
Language:
En
Journal:
Fish Shellfish Immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2010
Document type:
Article
Affiliation country:
Netherlands