Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution.
Cryobiology
; 61(3): 236-42, 2010 Dec.
Article
in En
| MEDLINE
| ID: mdl-20727872
ABSTRACT
INTRODUCTION:
Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage.METHODS:
Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a 'back table' intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES (n=6 per group) Group 1, 0%; Group 2, 2.5%; Group 3, 5%; Group 4, 10%. Energetics, oxidative stress, and morphology were assessed over a 24h time-course of cold storage.RESULTS:
Overall, the 5% HES solution, Group 3, demonstrated superior energetic status (ATP and total adenylates) compared to all groups, P<0.05. Malondialdehyde levels indicated a reduction in oxidative stress in Groups 3 and 4 (P<0.05). After 12h, median modified Parks' grades for Groups 2 and 3 were significantly lower than Groups 1 and 4, P<0.05.CONCLUSION:
Our data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hydroxyethyl Starch Derivatives
/
Organ Preservation Solutions
/
Intestine, Small
Limits:
Animals
Language:
En
Journal:
Cryobiology
Year:
2010
Document type:
Article
Affiliation country:
Canada