A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes.
Int Immunopharmacol
; 10(11): 1354-60, 2010 Nov.
Article
in En
| MEDLINE
| ID: mdl-20727998
ABSTRACT
Glycophorin A (GPA, CD235a) is a major membrane glycoprotein and marker of cells of the erythroid lineage. It is also the target of Plasmodium falciparum and of influenza virus. We describe a novel series of 10 antibodies towards GPA, recognizing four extra- and intracellular peptide epitopes of this molecule (defined by epitope mapping) and one mixed peptide/carbohydrate epitope. All antibodies bind better to the desialylated than to the fully sialylated molecule, including those specific for the intracellular epitope. For some of the antibodies (representing all five epitopes) functional binding constants were determined by Surface Plasmon Resonance. The new panel complements the already known anti-glycophorin antibodies and offers several potential applications for, e.g., differential diagnosis of erythroleukemias, lineage analyses of erythroid cells, isolation of senescent erythrocytes, or a highly sensitive neuraminidase assay.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glycophorins
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
Language:
En
Journal:
Int Immunopharmacol
Journal subject:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Germany