Multi-walled carbon nanotubes induce oxidative stress and apoptosis in human lung cancer cell line-A549.
Nanotoxicology
; 5(2): 195-207, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-20804439
ABSTRACT
Multi-walled carbon-nanotubes (MWCNTs)-induced apoptotic changes were studied in human lung epithelium cell line-A549. Non-cytotoxic doses of MWCNTs were identified using tetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) release assays. Cells were exposed to MWCNTs (0.5-100 µg/ml) for 6-72 h. Internalization and characterization of CNTs was performed by electron microscopy. Apoptotic changes were estimated by nuclear condensation, DNA laddering, and confirmed by expression of associated markers p(53), p(21WAF1/CIP1), Bax, Bcl(2) and activated caspase-3. MWCNTs induced the production of reactive oxygen species and malondialdehyde along with significant decrease in the activity of catalase and glutathione. MWCNTs-induced ROS generation was found not to be associated with the mitochondrial activity. In general, the changes were significant at 10 and 50 µg/ml only. Results indicate the involvement of oxidative stress and apoptosis in A549 cells exposed to MWCNTs. Our studies provide insights of the mechanisms involved in MWCNTs-induced apoptosis at cellular level.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Oxidative Stress
/
Nanotubes, Carbon
/
Cell Line, Tumor
Limits:
Animals
/
Humans
Language:
En
Journal:
Nanotoxicology
Journal subject:
TOXICOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
India