Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings.
Bioresour Technol
; 102(2): 703-9, 2011 Jan.
Article
in En
| MEDLINE
| ID: mdl-20843682
ABSTRACT
A root nodule bacterium, Sinorhizobium meliloti CCNWSX0020, resistant to 1.4 mM Cu2+ was isolated from Medicago lupulina growing in mine tailings. In medium supplied with copper, this bacterium showed cell deformation and aggregation due to precipitation of copper on the cell surface. Genes similar to the copper-resistant genes, pcoR and pcoA from Escherichia coli, were amplified by PCR from a 1.4-Mb megaplasmid. Inoculation with S. meliloti CCNWSX0020 increased the biomass of M. lupulina grown in medium added 0 and 100 mg Cu2+ kg(-1) by 45.8% and 78.2%, respectively, and increased the copper concentration inside the plant tissues grown in medium supplied with 100 µM Cu2+ by 39.3%, demonstrating that it is a prospective symbiotic system for bioremediation purposes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Symbiosis
/
Waste Products
/
Sinorhizobium meliloti
/
Copper
/
Medicago
/
Mining
Type of study:
Prognostic_studies
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2011
Document type:
Article
Affiliation country:
China
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM