CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin.
Biosens Bioelectron
; 26(3): 1109-13, 2010 Nov 15.
Article
in En
| MEDLINE
| ID: mdl-20850960
ABSTRACT
A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5-500 ngmL(-1) of BSA-OP, with a detection limit of 0.5 ngmL(-1). It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serum Albumin, Bovine
/
Quantum Dots
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
Thailand