Construction of an artificial glutathione peroxidase active site on copolymer vesicles.
Macromol Biosci
; 10(12): 1505-16, 2010 Dec 08.
Article
in En
| MEDLINE
| ID: mdl-20857392
ABSTRACT
To construct an efficient GPx mimic, a novel method for preparing polymer-based vesicles carrying GPx-active sites was developed. A series of block copolymers loaded with recognition and catalytic sites were synthesized based on polystyrene-block-poly[tri(ethylene glycol) methyl ether acrylate]s (PS-PMEO(3) MAs). By altering the molar ratio of the functional copolymers, vesicles with GPx activity were obtained by self-assembly of these functional copolymers through blending. The optimum GPx mimic constructed by the blending process exhibited high catalytic activity and acted as a real catalyst with typical saturation kinetics behavior. The method may be of benefit for designing other enzyme mimics and may cast a light on constructing other biologically related functional nanoparticles.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Engineering
/
Catalytic Domain
/
Glutathione Peroxidase
Language:
En
Journal:
Macromol Biosci
Journal subject:
BIOQUIMICA
Year:
2010
Document type:
Article