Amides/pharmacology; Hypertension, Pulmonary/drug therapy; Hypoxia/complications; Muscle, Smooth, Vascular/drug effects; Protein Kinase Inhibitors/pharmacology; Pyridines/pharmacology; Ventricular Dysfunction, Right/drug therapy; Ventricular Function, Right; Ventricular Remodeling/drug effects; rho-Associated Kinases/antagonists & inhibitors; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology; Age Factors; Aging; Amides/administration & dosage; Animals; Apoptosis/drug effects; Cell Proliferation/drug effects; Cells, Cultured; Chronic Disease; Disease Models, Animal; Endothelin-1/metabolism; Hemodynamics; Hypertension, Pulmonary/enzymology; Hypertension, Pulmonary/etiology; Hypertension, Pulmonary/physiopathology; Hypertrophy, Right Ventricular/enzymology; Hypertrophy, Right Ventricular/etiology; Hypertrophy, Right Ventricular/physiopathology; Hypertrophy, Right Ventricular/prevention & control; Hypoxia/enzymology; Hypoxia/physiopathology; Injections, Subcutaneous; Muscle, Smooth, Vascular/enzymology; Muscle, Smooth, Vascular/pathology; Myocardium/enzymology; Myocardium/pathology; Protein Kinase Inhibitors/administration & dosage; Pulmonary Artery/drug effects; Pulmonary Artery/enzymology; Pulmonary Artery/pathology; Pyridines/administration & dosage; RNA Interference; Rats; Receptor, Endothelin A/metabolism; Ventricular Dysfunction, Right/enzymology; Ventricular Dysfunction, Right/etiology; Ventricular Dysfunction, Right/physiopathology; rho-Associated Kinases/genetics; rho-Associated Kinases/metabolism