The aging-associated enzyme CLK-1 is a member of the carboxylate-bridged diiron family of proteins.
Biochemistry
; 49(45): 9679-81, 2010 Nov 16.
Article
in En
| MEDLINE
| ID: mdl-20923139
ABSTRACT
The aging-associated enzyme CLK-1 is proposed to be a member of the carboxylate-bridged diiron family of proteins. To evaluate this hypothesis and characterize the protein, we expressed soluble mouse CLK-1 (MCLK1) in Escherichia coli as a heterologous host. Using Mössbauer and EPR spectroscopy, we established that MCLK1 indeed belongs to this protein family. Biochemical analyses of the in vitro activity of MCLK1 with quinone substrates revealed that NADH can serve directly as a reductant for catalytic activation of dioxygen and substrate oxidation by the enzyme, with no requirement for an additional reductase protein component. The direct reaction of NADH with a diiron-containing oxidase enzyme has not previously been encountered for any member of the protein superfamily.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mitochondrial Proteins
/
Membrane Proteins
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Biochemistry
Year:
2010
Document type:
Article
Affiliation country:
United States