Transcutaneous immunization with hydrophilic recombinant gp100 protein induces antigen-specific cellular immune response.
Cell Immunol
; 266(1): 98-103, 2010.
Article
in En
| MEDLINE
| ID: mdl-20947070
ABSTRACT
The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In a mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ. Transcutaneous antigen delivery may provide a safe, simple and effective method to elicit cell-mediated immunity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Vaccination
/
Gp100 Melanoma Antigen
/
Hydrophobic and Hydrophilic Interactions
/
Immunity, Cellular
Language:
En
Journal:
Cell Immunol
Year:
2010
Document type:
Article
Affiliation country:
Israel