Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo.
Br J Nutr
; 105(2): 167-70, 2011 Jan.
Article
in En
| MEDLINE
| ID: mdl-21078213
ABSTRACT
Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL from oxidation, thereby exhibiting antiatherogenic properties. Using a luciferase reporter gene assay, we tested curcumin for its ability to induce PON1 in Huh7 hepatocytes in culture. Curcumin ( ≥ 10 µmol/l) dose-dependently induced PON1 transactivation in Huh7 cells. However, dietary supplementation of female B6C3F1 mice with curcumin (500 mg/kg diet) for 2 weeks did not increase the hepatic PON1 mRNA and protein levels. No curcumin was detectable in the plasma of the 12 h fasted mice. In conclusion, curcumin may be a potent PON1 inducer in cultured cells in vitro, but not in the liver of curcumin-fed mice because of its low concentrations in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Curcumin
/
Hepatocytes
/
Aryldialkylphosphatase
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Br J Nutr
Year:
2011
Document type:
Article
Affiliation country:
Germany