Organic solvent-free approach to single step fabrication of Eudragit nanoparticles using Labrasol.

ABSTRACT

The present investigation describes organic volatile solvent-free approach for the single step fabrication of Eudragit nanoparticles. The solubility of various grades of Eudragit viz. Eudragit L100-55, Eudragit L100, Eudragit S100, Eudragit EPO, Eudragit RSPO and Eudragit RLPO in Labrasol (Caprylocaproyl macrogol-8 glycerides) was determined. We observed that Labrasol has the ability to solubilize various cationic Eudragits such as Eudragit RSPO, Eudragit RLPO and Eudragit EPO at a concentration as high as 200 mg/ml. We also evaluated the ability of Labrasol to act as a nanoparticle stabilizer due to its amphiphilic nature and high HLB of 14. It was observed that Eudragit EPO, Eudragit RSPO and Eudragit RLPO nanoparticles of size 110-150 nm could be easily fabricated using Labrasol as a solubilizer and nanoparticle stabilizer. Transmission electron microscopy was carried out to confirm the size and morphology of the nanoparticles. We could encapsulate hydrophobic drugs such as repaglinide and triclosan in the Eudragit RLPO nanoparticles as Labrasol also had the ability to solubilize these hydrophobic drugs. The ability of Eudragit RSPO and RLPO nanoparticles to condense plasmid DNA was also established. This is the first report that demonstrates the polymer solubilizing potential of Labrasol.