Simultaneous calibration of optical tweezers spring constant and position detector response.
Opt Express
; 18(25): 26469-74, 2010 Dec 06.
Article
in En
| MEDLINE
| ID: mdl-21164997
ABSTRACT
We demonstrate a fast and direct calibration method for systems using a single laser for optical tweezers and particle position detection. The method takes direct advantage of back-focal-plane interferometry measuring not an absolute but a differential position, i.e. the position of the trapped particle relative to the center of the optical tweezers. Therefore, a fast step-wise motion of the optical tweezers yields the impulse response of the trapped particle. Calibration parameters such as the detector's spatial and temporal response and the spring constant of the optical tweezers then follow readily from fitting the measured impulse response.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Algorithms
/
Optical Tweezers
/
Interferometry
Type of study:
Prognostic_studies
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2010
Document type:
Article
Affiliation country:
France