Identification, cloning, heterologous expression, and characterization of a NADPH-dependent 7ß-hydroxysteroid dehydrogenase from Collinsella aerofaciens.

Liu, Luo; Aigner, Arno; Schmid, Rolf D

Liu, Luo; Aigner, Arno; Schmid, Rolf D.

Affiliation

Liu L; Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany. liuluo02@gmail.com

Appl Microbiol Biotechnol ; 90(1): 127-35, 2011 Apr.

Article in En | MEDLINE | ID: mdl-21181147

Subject(s)

Actinobacteria/enzymology; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Cloning, Molecular; Gene Expression; Hydroxysteroid Dehydrogenases/chemistry; Hydroxysteroid Dehydrogenases/genetics; NADP/metabolism; Actinobacteria/chemistry; Actinobacteria/classification; Actinobacteria/genetics; Amino Acid Sequence; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Enzyme Stability; Escherichia coli/genetics; Escherichia coli/metabolism; Hydroxysteroid Dehydrogenases/isolation & purification; Hydroxysteroid Dehydrogenases/metabolism; Molecular Sequence Data; Phylogeny; Sequence Alignment; Substrate Specificity; Ursodeoxycholic Acid/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Gene Expression / Cloning, Molecular / Actinobacteria / Hydroxysteroid Dehydrogenases / NADP Type of study: Diagnostic_studies Language: En Journal: Appl Microbiol Biotechnol Year: 2011 Document type: Article Affiliation country: Germany Country of publication: Germany

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