Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method.
Proteomics
; 11(2): 324-8, 2011 Jan.
Article
in En
| MEDLINE
| ID: mdl-21204259
ABSTRACT
Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organometallic Compounds
/
Electrophoresis, Gel, Two-Dimensional
/
Proteins
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Proteomics
Journal subject:
BIOQUIMICA
Year:
2011
Document type:
Article
Affiliation country:
France