Mechanisms of ceftazidime and ciprofloxacin transport through porins in multidrug-resistance developed by extended-spectrum beta-lactamase E.coli strains.
J Fluoresc
; 21(4): 1421-9, 2011 Jul.
Article
in En
| MEDLINE
| ID: mdl-21234661
ABSTRACT
Resistance towards antibiotics stands out today as a major issue in the clinical act of treatment of bacterial-generated infections. This process was characterized in proteoliposomes reconstituted from an E.coli strain isolated from invasive infections (blood culture) occurred in patients with a cardio-vascular device admitted for surgery. Fluorescence spectroscopy and patch-clamp technique have been used. Two types of antibiotics have been targeted ceftazidime and ciprofloxacin. Antibiotics addition in proteoliposomes suspension undergoes a quenching in tryptophan residues from outer membrane porins structure, probably due to the formation of a transient non-fluorescent porin-antibiotic complex. Patch-clamp recordings revealed strong ion current blockages for both antibiotics, reflecting antibiotic-channel interactions but with varying strength of interaction. The present study puts forward the mechanism of multidrug-resistance in extended-spectrum beta-lactamase E.coli strains, as being caused by alterations of the antibiotics transport across the porins of the outer bacterial membrane.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ciprofloxacin
/
Ceftazidime
/
Porins
/
Drug Resistance, Multiple, Bacterial
/
Escherichia coli
/
Beta-Lactamase Inhibitors
/
Anti-Bacterial Agents
Limits:
Humans
Language:
En
Journal:
J Fluoresc
Journal subject:
BIOFISICA
Year:
2011
Document type:
Article
Affiliation country:
Romania