Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association.
Biochimie
; 93(4): 725-9, 2011 Apr.
Article
in En
| MEDLINE
| ID: mdl-21237242
ABSTRACT
Among 4.5 thousand nucleotides of Escherichia coli ribosome 36 are modified. These nucleotides are clustered in the functional centers of ribosome, particularly on the interface of large and small subunits. Nucleotide m(2)G1835 located on the 50S side of intersubunit bridge cluster B2 is modified by N2-methyltransferase RlmG. By means of isothermal titration calorimetry and Rayleigh light scattering, we have found that methylation of m(2)G1835 specifically enhances association of ribosomal subunits. No defects in fidelity of translation or interaction with translation GTPases could be ascribed to the ribosomes unmethylated at G1835 of the 23S rRNA. Methylation of G1835 was found to provide a significant advantage for bacteria at osmotic and oxidative stress.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribosomes
/
RNA, Ribosomal, 23S
/
Escherichia coli
Type of study:
Risk_factors_studies
Language:
En
Journal:
Biochimie
Year:
2011
Document type:
Article
Affiliation country:
RUSSIA