[Integration sites in HCC biopsy].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 24(5): 386-8, 2010 Oct.
Article
in Zh
| MEDLINE
| ID: mdl-21280333
ABSTRACT
OBJECTIVE:
To compare the performance of Inverse-PCR, Alu-PCR and Cassette-ligation-mediated PCR (CLM-PCR) in HBV DNA integration sites identification.METHODS:
One HCC biopsy was obtained from surgically resected sample. The patient was positive for serum hepatitis B surface antigen (HBsAg). The genomic DNA was purified by the standard phenol/chloroform extraction and ethanol precipitation method. Seperated set of primers were designed to amplify the HBV DNA integration region by means of 3 different PCR methods respectively. The PCR products were analyzed by electrophoresis, then cloned to PMD18-T vector for DNA sequencing. The sequence alignment was performed under Blast software.RESULTS:
7 bands and 22 sequencing results was obtained from IPCR and 3 integration sites was identified. Alu-PCR provided 12 bands and 32 sequencing results, and CLM-PCR showed 12 bands and 4 sequencing results. No integration site was identified from the latter two.CONCLUSION:
IPCR compared with another two methods showed a reliable capacity in HBV DNA integration site identification.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hepatitis B virus
/
Polymerase Chain Reaction
/
Virus Integration
/
Carcinoma, Hepatocellular
/
Liver Neoplasms
Limits:
Adult
/
Humans
/
Male
Language:
Zh
Journal:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Journal subject:
VIROLOGIA
Year:
2010
Document type:
Article
Affiliation country:
China