Flow-based autocorrelation studies for the detection and investigation of single-particle surface-enhanced resonance Raman spectroscopic events.
Anal Chem
; 83(4): 1418-24, 2011 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-21314203
ABSTRACT
We report on the characterization and detection of single metallic nanoparticles using a combination of correlation spectroscopy and surface-enhanced resonance Raman spectroscopy (SERRS). Minimizing the number of independent characterization steps is critical to efficiently perform such an analysis. In this article, we improve upon conventional diffusion-limited approaches by implementing a flow-based system with high temporal resolution detection. The benefits of flow over diffusion measurements allow for a higher throughput of detected events resulting in shorter analysis times. The nanoparticles are sized using their rotational diffusion time calculated with a modified autocorrelation function. Experiments are performed using Au nanoparticles labeled with the reporter molecule malachite green isothiocyanate on a custom-built Raman spectrometer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Diagnostic_studies
Language:
En
Journal:
Anal Chem
Year:
2011
Document type:
Article
Affiliation country:
United kingdom