How Förster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology.
Chemphyschem
; 12(3): 442-61, 2011 Feb 25.
Article
in En
| MEDLINE
| ID: mdl-21328516
ABSTRACT
Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteins
/
Protein Interaction Mapping
/
Fluorescence Resonance Energy Transfer
/
Neoplasms
Limits:
Humans
Language:
En
Journal:
Chemphyschem
Journal subject:
BIOFISICA
/
QUIMICA
Year:
2011
Document type:
Article
Affiliation country:
United kingdom