Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis.
Biotechnol Lett
; 33(6): 1209-13, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21331586
ABSTRACT
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xylitol
/
Candida tropicalis
/
D-Xylulose Reductase
Language:
En
Journal:
Biotechnol Lett
Year:
2011
Document type:
Article