A biosensor for the determination of ß-galactosidase activity: a different viewpoint on biosensors.

ABSTRACT

ß-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor ß-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. ß-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for ß-galactosidase detection was in the range of 0.0118 U mL(-1)to 0.47 U mL(-1)and a shorter response time of ∼50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of ß-galactosidase activity.