Dextran-coated silica nanoparticles for calcium-sensing.
Analyst
; 136(8): 1722-7, 2011 Apr 21.
Article
in En
| MEDLINE
| ID: mdl-21369598
ABSTRACT
This article describes the synthesis and characterisation of fluorescent composite nanoparticles consisting of a silica core and a dextran shell. The silica core contains a rhodamine-based reference dye, which allows ratiometric measurements and the dextran shell is labelled with the Ca(2+)-sensitive dye Fluo-4. The nanoparticles have an average hydrodynamic diameter of 95 nm, good colloidal stability and show a 2.9-fold increase in fluorescence intensity upon binding to Ca(2+) ions. The apparent dissociation constant of K'(d) ≈ 520 nM is well suited for measurements in the physiological range.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrometry, Fluorescence
/
Calcium
/
Dextrans
/
Silicon Dioxide
/
Nanoparticles
Language:
En
Journal:
Analyst
Year:
2011
Document type:
Article
Affiliation country:
Ireland