DNA barcoding Indian marine fishes.
Mol Ecol Resour
; 11(1): 60-71, 2011 Jan.
Article
in En
| MEDLINE
| ID: mdl-21429101
ABSTRACT
DNA barcoding has been adopted as a global bio-identification system for animals in recent years. A major national programme on DNA barcoding of fish and marine life was initiated in India by the authors during 2006 and 115 species of marine fish covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids and Silurids representing 79 Genera and 37 Families from the Indian Ocean have been barcoded for the first time using cytochrome c oxidase I gene (COI) of the mtDNA. The species were represented by multiple specimens and a total of 397 sequences were generated. After amplification and sequencing of 707 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two parameter (K2P) distances within species, genera, families, orders were 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode-based species identification system, phylogenetic relationships among the species have also been attempted. The neighbour-joining tree revealed distinct clusters in concurrence with the taxonomic status of the species.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
DNA Barcoding, Taxonomic
/
Fishes
Type of study:
Evaluation_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Mol Ecol Resour
Year:
2011
Document type:
Article
Affiliation country:
India