Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay.
J Clin Microbiol
; 49(6): 2259-61, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21490185
ABSTRACT
A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas aeruginosa
/
Blood
/
Bacteriological Techniques
/
In Situ Hybridization, Fluorescence
/
Peptide Nucleic Acids
/
Escherichia coli
/
Klebsiella pneumoniae
Type of study:
Clinical_trials
/
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2011
Document type:
Article
Affiliation country:
United States
