[Expression of vigilin in cell lines and human hepatocellular carcinoma].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 42(2): 170-3, 2011 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-21500547
ABSTRACT
OBJECTIVE:
To explore the expression status of Vigilin (high density lipoprotein binding protein) in various cell lines and human hepatocellular carcinoma (HCC) tissues.METHODS:
The expression of Vigilin was measured semiquantitatively with western blot in hepatic cancer, cervical cancer and normal cell lines. The samples of 59 hepatocellular carcinoma tissues, 59 adjacent liver tissues and 33 distant non-tumor liver tissues were collected, Vigilin expression in the above samples was detected by immunohistochemistry.RESULTS:
Vigilin expressed in all cell lines, but no expression was found in peripheral blood lymphocytes. The expression levels of Vigilin in tumor cell lines were higher than those in normal cell lines (P < 0.05). Most of the hepatic cells expressed Vigilin, but the expression levels were different (tumor tissues 0.2226 +/- 0.054, adjacent tissues 0.2060 +/- 0.056, distant tissues 0.1820 +/- 0.038, P < 0.001). Highly expression of Vigilin was observed in 54% of tumor tissues, 35% adjacement tissues, and 6% of distant non-tumor tissues, respectively.CONCLUSION:
Vigilin may have relationship with HCC progression and proliferation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA-Binding Proteins
/
Carcinoma, Hepatocellular
/
Liver Neoplasms
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
Zh
Journal:
Sichuan Da Xue Xue Bao Yi Xue Ban
Year:
2011
Document type:
Article
Affiliation country:
China