Human fat grafting alleviates radiation skin damage in a murine model.
Plast Reconstr Surg
; 128(2): 363-372, 2011 Aug.
Article
in En
| MEDLINE
| ID: mdl-21502909
ABSTRACT
BACKGROUND:
Autogenous fat grafting has been observed to alleviate the sequelae of chronic radiodermatitis. To date, no study has replicated this finding in an animal model.METHODS:
The dorsa of adult wild-type FVB mice were shaved and depilated. The dorsal skin was then distracted away from the body and irradiated (45 Gy). Four weeks after irradiation, 1.5-cc fat or sham grafts were placed in the dorsal subcutaneous space. Gross results were analyzed photometrically. The animals were euthanized at 4 and 8 weeks after fat or sham grafting and their dorsal skin was processed for histologic analysis.RESULTS:
Hyperpigmentation and ulceration were grossly improved in fat-grafted mice compared with sham-grafted controls. This improvement manifested histologically in a number of ways. For example, epidermal thickness measurements demonstrated decreased thickness in fat-grafted animals at both time points (20.6 ± 1.5 µm versus 55.2 ± 5.6 µm, p = 0.004; 17.6 ± 1.1 µm versus 36.3 ± 6.1 µm, p = 0.039). Picrosirius red staining demonstrated a diminished scar index in fat-treated animals at both time points as well (0.54 ± 0.05 versus 0.74 ± 0.07, p = 0.034; and 0.55 ± 0.06 versus 0.93 ± 0.07, p = 0.001).CONCLUSION:
Fat grafting attenuates inflammation in acute radiodermatitis and slows the progression of fibrosis in chronic radiodermatitis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiodermatitis
/
Skin
/
Adipose Tissue
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Plast Reconstr Surg
Year:
2011
Document type:
Article
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA