Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids.

Takimoto, Jeffrey K; Dellas, Nikki; Noel, Joseph P; Wang, Lei

Takimoto, Jeffrey K; Dellas, Nikki; Noel, Joseph P; Wang, Lei.

Affiliation

Takimoto JK; The Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United States.

ACS Chem Biol ; 6(7): 733-43, 2011 Jul 15.

Article in En | MEDLINE | ID: mdl-21545173

Subject(s)

Amino Acyl-tRNA Synthetases/chemistry; Amino Acyl-tRNA Synthetases/metabolism; Lysine/analogs & derivatives; Amino Acid Sequence; Amino Acid Substitution; Amino Acyl-tRNA Synthetases/genetics; Aminoacylation; Catalytic Domain; Crystallography, X-Ray; Directed Molecular Evolution; Escherichia coli/metabolism; HeLa Cells; Humans; Lysine/chemistry; Lysine/metabolism; Methanosarcina/enzymology; Methyltyrosines/chemistry; Methyltyrosines/metabolism; Molecular Sequence Data; Mutation; Protein Conformation; Structure-Activity Relationship; Substrate Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amino Acyl-tRNA Synthetases / Lysine Limits: Humans Language: En Journal: ACS Chem Biol Year: 2011 Document type: Article Affiliation country: United States Country of publication: United States

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