Development of filtration-based time-resolved fluorescence assay for the high-throughput screening of urotensin II receptor antagonist.

Oh, Kwang-Seok; Lee, Sunghou; Lee, Byung Ho

Oh, Kwang-Seok; Lee, Sunghou; Lee, Byung Ho.

Affiliation

Oh KS; Bio-Organic Science Division, Korea Research Institute of Chemical Technology Yuseong, Daejeon, Republic of Korea.

Assay Drug Dev Technol ; 9(5): 514-21, 2011 Oct.

Article in En | MEDLINE | ID: mdl-21561377

Subject(s)

Drug Evaluation, Preclinical; Europium/chemistry; Fluorescence Polarization/methods; High-Throughput Screening Assays/methods; Sulfonamides/pharmacology; Urotensins/antagonists & inhibitors; Animals; Binding, Competitive; Biological Assay; Drug Discovery; Fluorescence; HEK293 Cells; Humans; Inhibitory Concentration 50; Ligands; Mice; Molecular Targeted Therapy; Protein Binding; Radioisotopes; Radioligand Assay; Rats; Reproducibility of Results; Sensitivity and Specificity; Sulfonamides/analysis; Sulfonamides/chemical synthesis; Time Factors; Urotensins/genetics; Urotensins/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfonamides / Urotensins / Drug Evaluation, Preclinical / Europium / High-Throughput Screening Assays / Fluorescence Polarization Type of study: Diagnostic_studies / Screening_studies Limits: Animals / Humans Language: En Journal: Assay Drug Dev Technol Journal subject: FARMACOLOGIA Year: 2011 Document type: Article Country of publication: United States

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