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Iron quantification of microbleeds in postmortem brain.
McAuley, Grant; Schrag, Matthew; Barnes, Samuel; Obenaus, Andre; Dickson, April; Holshouser, Barbara; Kirsch, Wolff.
Affiliation
  • McAuley G; Neurosurgery Center for Research, Training and Education, Loma Linda University, Loma Linda, California 92354, USA.
Magn Reson Med ; 65(6): 1592-601, 2011 Jun.
Article in En | MEDLINE | ID: mdl-21590801
Brain microbleeds (BMB) are associated with chronic and acute cerebrovascular disease and present a source of pathologic iron to the brain proportional to extravasated blood. Therefore, BMB iron content is potentially a valuable biomarker. We tested noninvasive phase image methods to quantify iron content and estimate true source diameter (i.e., unobscured by the blooming effect) of BMB in postmortem human tissue. Tissue slices containing BMB were imaged using a susceptibility weighted imaging protocol at 11.7T. BMB lesions were assayed for iron content using atomic absorption spectrometry. Measurements of geometric features in phase images were related to lesion iron content and source diameter using a mathematical model. BMB diameter was estimated by image feature geometry alone without explicit relation to the magnetic susceptibility. A strong linear relationship (R(2) = 0.984, P < 0.001) predicted by theory was observed in the experimental data, presenting a tentative standardization curve where BMB iron content in similar tissues could be calculated. In addition, we report BMB iron mass measurements, as well as upper bound diameter and lower bound iron concentration estimates. Our methods potentially allows the calculation of brain iron load indices based on BMB iron content and classification of BMB by size unobscured by the blooming effect.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Magnetic Resonance Imaging / Intracranial Hemorrhages / Iron Type of study: Prognostic_studies Limits: Humans Language: En Journal: Magn Reson Med Journal subject: DIAGNOSTICO POR IMAGEM Year: 2011 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Magnetic Resonance Imaging / Intracranial Hemorrhages / Iron Type of study: Prognostic_studies Limits: Humans Language: En Journal: Magn Reson Med Journal subject: DIAGNOSTICO POR IMAGEM Year: 2011 Document type: Article Affiliation country: United States Country of publication: United States