Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae).
Am J Bot
; 97(5): e26-30, 2010 May.
Article
in En
| MEDLINE
| ID: mdl-21622433
ABSTRACT
PREMISE OF THE STUDY Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds. METHODS AND RESULTS:
Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n-enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively.CONCLUSIONS:
Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Am J Bot
Year:
2010
Document type:
Article
Affiliation country:
Spain