A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals.
Acta Trop
; 120(1-2): 40-5, 2011.
Article
in En
| MEDLINE
| ID: mdl-21664340
ABSTRACT
The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/µl). The sensitivity threshold was 0.2 parasites/µl and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Plasmodium vivax
/
DNA, Protozoan
/
Parasitemia
/
Real-Time Polymerase Chain Reaction
/
Malaria
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Acta Trop
Year:
2011
Document type:
Article
Affiliation country:
South Korea