Bioanalytical approaches to analyzing peptides and proteins by LC--MS/MS.
Bioanalysis
; 3(12): 1379-97, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21679032
ABSTRACT
Peptides and proteins have been utilized as therapeutic agents for over 40 years. Traditional approaches to quantify these molecules in biological matrices have utilized immunoassay approaches that can be time inefficient, lack assay specificity and have limited analytical ranges. The advances in sample preparation technologies, chromatographic systems and their chemistries, mass spectrometers and their software over the last decade have meant that LC-MS/MS approaches to peptide and protein quantification are feasible and can overcome the problems associated with quantification by immunoassay. In this article we present an overview of the challenges and approaches to overcome them when performing quantitative bioanalysis of peptides and proteins by LC-MS/MS.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Pharmaceutical Preparations
/
Proteins
/
Chromatography, Liquid
/
Tandem Mass Spectrometry
Limits:
Humans
Language:
En
Journal:
Bioanalysis
Year:
2011
Document type:
Article