Inhibiting effect of bioactive metabolites produced by mushroom cultivation on bacterial quorum sensing-regulated behaviors.
Chemotherapy
; 57(4): 292-7, 2011.
Article
in En
| MEDLINE
| ID: mdl-21778717
ABSTRACT
AIMS:
This study aimed to search for novel quorum sensing (QS) inhibitors from mushroom and to analyze their inhibitory activity, with a view to their possible use in controlling detrimental infections.METHODS:
The bioactive metabolites produced by mushroom cultivation were tested for their abilities to inhibit QS-regulated behavior. All mushroom strains were cultivated in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2 vol by freeze-drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader.RESULTS:
The results have revealed that, of 102 mushroom strains, the bioactive metabolites produced by 14 basidiomycetes were found to inhibit violacein production, a QS-regulated behavior in C. violaceum.CONCLUSIONS:
Higher fungi can produce QS-inhibitory compounds.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromobacterium
/
Quorum Sensing
/
Fungi
/
Anti-Bacterial Agents
Language:
En
Journal:
Chemotherapy
Year:
2011
Document type:
Article