Effect of cystamine on blood pressure and vascular characteristics in spontaneously hypertensive rats.
J Vasc Res
; 48(6): 476-84, 2011.
Article
in En
| MEDLINE
| ID: mdl-21778764
ABSTRACT
BACKGROUND:
Tissue transglutaminase (t-TG) has been implicated in small artery remodelling. The aim of this study was to determine if cystamine, an inhibitor of t-TG, could reduce blood pressure in spontaneously hypertensive rats (SHR) and if so to what extent this is mediated through small arteries.METHODS:
In vitro inhibition of t-TG, with cystamine, was studied in organ culture and wire myograph setups in small mesenteric arteries obtained from SHR. In vivo treatment with cystamine (80 mg/kg/day) or amlodipine (10 mg/kg/day) was performed with osmotic pumps in adult SHR, and hemodynamic parameters determined with telemetry. Plasma concentrations of cystamine were determined with a liquid chromatography setup. Small arteries were harvested following administration of cystamine, and structural as well as functional characteristics were determined.RESULTS:
SHR small arteries showed inward remodelling following in vitro activation. Administration of cystamine caused attenuation of the inward remodelling induced by activation. In vivo administration of cystamine caused a 9 ± 2 mm Hg reduction in blood pressure, but with no detectable alterations in small artery structure.CONCLUSION:
t-TG is potentially involved in vascular remodelling of SHR small arteries and results support a possible role for t-TG in blood pressure control.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Pressure
/
Transglutaminases
/
GTP-Binding Proteins
/
Cystamine
/
Enzyme Inhibitors
/
Hypertension
/
Mesenteric Arteries
Limits:
Animals
Language:
En
Journal:
J Vasc Res
Journal subject:
ANGIOLOGIA
Year:
2011
Document type:
Article