Combination of site-directed mutagenesis and yeast surface display enhances Rhizomucor miehei lipase esterification activity in organic solvent.

Han, Shuang-yan; Zhang, Jun-hui; Han, Zhen-lin; Zheng, Sui-ping; Lin, Ying

Han, Shuang-yan; Zhang, Jun-hui; Han, Zhen-lin; Zheng, Sui-ping; Lin, Ying.

Affiliation

Han SY; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People's Republic of China.

Biotechnol Lett ; 33(12): 2431-8, 2011 Dec.

Article in En | MEDLINE | ID: mdl-21809089

Subject(s)

Genetic Enhancement/methods; Lipase/chemistry; Mutagenesis, Site-Directed/methods; Organic Chemicals/chemistry; Rhizomucor/enzymology; Rhizomucor/genetics; Solvents/chemistry; Enzyme Activation; Enzyme Stability; Esterification; Lipase/metabolism; Protein Engineering/methods

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organic Chemicals / Solvents / Mutagenesis, Site-Directed / Rhizomucor / Genetic Enhancement / Lipase Language: En Journal: Biotechnol Lett Year: 2011 Document type: Article

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