Quantification of ligand PET studies using a reference region with a displaceable fraction: application to occupancy studies with [(11)C]-DASB as an example.
J Cereb Blood Flow Metab
; 32(1): 70-80, 2012 Jan.
Article
in En
| MEDLINE
| ID: mdl-21811290
ABSTRACT
This paper aims to build novel methodology for the use of a reference region with specific binding for the quantification of brain studies with radioligands and positron emission tomography (PET). In particular (1) we introduce a definition of binding potential BP(D)=DVR-1 where DVR is the volume of distribution relative to a reference tissue that contains ligand in specifically bound form, (2) we validate a numerical methodology, rank-shaping regularization of exponential spectral analysis (RS-ESA), for the calculation of BP(D) that can cope with a reference region with specific bound ligand, (3) we demonstrate the use of RS-ESA for the accurate estimation of drug occupancies with the use of correction factors to account for the specific binding in the reference. [(11)C]-DASB with cerebellum as a reference was chosen as an example to validate the methodology. Two data sets were used; four normal subjects scanned after infusion of citalopram or placebo and further six test-retest data sets. In the drug occupancy study, the use of RS-ESA with cerebellar input plus corrections produced estimates of occupancy very close the ones obtained with plasma input. Test-retest results demonstrated a tight linear relationship between BP(D) calculated either with plasma or with a reference input and high reproducibility.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfides
/
Cerebellum
/
Radiopharmaceuticals
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Positron-Emission Tomography
/
Aniline Compounds
Type of study:
Clinical_trials
/
Prognostic_studies
Limits:
Adult
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Cereb Blood Flow Metab
Year:
2012
Document type:
Article
Affiliation country:
United kingdom