Simultaneous suppression of TGF-ß and ERK signaling contributes to the highly efficient and reproducible generation of mouse embryonic stem cells from previously considered refractory and non-permissive strains.

Hassani, Seyedeh-Nafiseh; Totonchi, Mehdi; Farrokhi, Ali; Taei, Adeleh; Larijani, Mehran Rezaei; Gourabi, Hamid; Baharvand, Hossein

Hassani, Seyedeh-Nafiseh; Totonchi, Mehdi; Farrokhi, Ali; Taei, Adeleh; Larijani, Mehran Rezaei; Gourabi, Hamid; Baharvand, Hossein.

Affiliation

Hassani SN; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395-4644, Tehran, Iran.

Stem Cell Rev Rep ; 8(2): 472-81, 2012 Jun.

Article in En | MEDLINE | ID: mdl-21814763

Subject(s)

Cell Culture Techniques/methods; Embryonic Stem Cells/enzymology; Extracellular Signal-Regulated MAP Kinases/metabolism; MAP Kinase Signaling System; Transforming Growth Factor beta/metabolism; Animals; Benzamides/pharmacology; Biomarkers/metabolism; Blastocyst/cytology; Blastocyst/drug effects; Blastocyst/metabolism; Cell Differentiation/drug effects; Cell Line; Culture Media/pharmacology; Dioxoles/pharmacology; Diphenylamine/analogs & derivatives; Diphenylamine/pharmacology; Embryonic Stem Cells/cytology; Embryonic Stem Cells/drug effects; Gene Expression Regulation/drug effects; MAP Kinase Signaling System/drug effects; Mice; Mice, Inbred Strains; Pluripotent Stem Cells/cytology; Pluripotent Stem Cells/drug effects; Pluripotent Stem Cells/metabolism; Reproducibility of Results; Small Molecule Libraries/pharmacology; Time Factors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transforming Growth Factor beta / Cell Culture Techniques / MAP Kinase Signaling System / Extracellular Signal-Regulated MAP Kinases / Embryonic Stem Cells Type of study: Prognostic_studies Limits: Animals Language: En Journal: Stem Cell Rev Rep Year: 2012 Document type: Article Affiliation country: Iran

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