Detection of immunoglobulins G and M to rubella virus by time-resolved immunofluorometry.
J Clin Microbiol
; 28(3): 573-9, 1990 Mar.
Article
in En
| MEDLINE
| ID: mdl-2182672
ABSTRACT
We describe new methods for the detection of immunoglobulin G (IgG) and IgM rubella-specific antibodies in serum. The IgG assay was based on a solid-phase rubella antigen immobilization approach, and the IgM assay was based on the IgM capture assay principle. Both assays used biotinylated antibodies (anti-human IgG and antirubella monoclonal antibody, respectively). The tracer system was based on streptavidin labeled with a fluorescent europium chelate. The final measurements were done by using time-resolved fluorescence. Both assays were thoroughly evaluated with clinical samples and compared successfully with established techniques. We anticipate that these assays are suitable for routine clinical use.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rubella virus
/
Immunoglobulin G
/
Immunoglobulin M
/
Antibodies, Viral
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
1990
Document type:
Article
Affiliation country:
Canada