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Successful establishment of an orthotopic hepatoblastoma in vivo model in NOD/LtSz-scid IL2Rγnull mice.
Ellerkamp, Verena; Armeanu-Ebinger, Sorin; Wenz, Julia; Warmann, Steven W; Schäfer, Juergen; Ruck, Peter; Fuchs, Joerg.
Affiliation
  • Ellerkamp V; Department of Pediatric Surgery, Children's University Hospital Tuebingen, Tuebingen, Germany. verenaellerkamp@googlemail.com
PLoS One ; 6(8): e23419, 2011.
Article in En | MEDLINE | ID: mdl-21853130
Investigation of hepatoblastoma in experimental conditions contributes relevantly to a detailed understanding of tumor biology and the investigation of new treatment approaches. Most systematical analyses currently use subcutaneous xenografts. We established a reproducible intrahepatic model with the hepatoblastoma-cell lines HuH6 and HepT1. The cells were stably transfected with a plasmid vector encoding for Gaussia luciferase. HuH6 and HepT1 were injected intrasplenically in NOD/LtSz-scid IL2Rγnull mice. Mice were splenectomized in order to avoid intrasplenical tumor growth. Multifocal intrahepatic tumor growth was observed in 85% (11/13) of HuH6 tumors and 55% (5/9) of HepT1 tumors. Serum Alpha-fetoprotein and Gaussia luciferase increased 5 weeks after tumor-cell inoculation. Tumors were detected by MRI at this time point. Immunhistochemical analysis such as vascularity (CD31), proliferation index (Ki-67), cytokeratin 7 and distribution of ß-catenin in intrahepatic tumors were different to subcutaneous tumors. We established a reproducible xenograft model for intrahepatic hepatoblastoma growth with a high tumor incidence. Monitoring of tumor cell viability was optimized by measuring GLuc. This model enables further experimental investigations of HB in a more physiological milieu as emphasized by the ß-catenin distribution.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hepatoblastoma / Disease Models, Animal / Interleukin Receptor Common gamma Subunit / Liver Neoplasms Limits: Animals / Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2011 Document type: Article Affiliation country: Germany Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hepatoblastoma / Disease Models, Animal / Interleukin Receptor Common gamma Subunit / Liver Neoplasms Limits: Animals / Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2011 Document type: Article Affiliation country: Germany Country of publication: United States