A human cell extract-based assay for the activation of ATM and ATR checkpoint kinases.

Shiotani, Bunsyo; Zou, Lee

Shiotani, Bunsyo; Zou, Lee.

Affiliation

Shiotani B; Harvard Medical School, Massachusetts General Hospital Cancer Center, Charlestown, MA, USA. bshiotani@partners.org

Methods Mol Biol ; 782: 181-91, 2011.

Article in En | MEDLINE | ID: mdl-21870292

Subject(s)

Cell Cycle Proteins/metabolism; DNA-Binding Proteins/metabolism; Enzyme Assays/methods; Protein Serine-Threonine Kinases/metabolism; Tumor Suppressor Proteins/metabolism; Ataxia Telangiectasia Mutated Proteins; Blotting, Western; Cell Cycle Proteins/genetics; DNA Breaks, Double-Stranded; DNA-Binding Proteins/genetics; Electrophoresis, Polyacrylamide Gel; HeLa Cells; Humans; Phosphorylation; Protein Serine-Threonine Kinases/genetics; Tumor Suppressor Proteins/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Serine-Threonine Kinases / Cell Cycle Proteins / Tumor Suppressor Proteins / DNA-Binding Proteins / Enzyme Assays Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2011 Document type: Article Affiliation country: United States

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