Cationic polydiacetylene micelles for gene delivery.
Bioconjug Chem
; 22(10): 1916-23, 2011 Oct 19.
Article
in En
| MEDLINE
| ID: mdl-21923152
ABSTRACT
Cationic surfactants easily interact with plasmid DNA to form small lipoplexes. However, their detergent behavior and associated biological toxicity limit their use as gene delivery vectors. We have incorporated a diacetylene motif in the hydrophobic chain of cationic surfactants. By using UV irradiation, the small cationic micelles (9 nm) obtained with diacetylenic detergents were photopolymerized into 40 nm spheres. Electrostatic interactions with plasmid DNA led to the formation of 45 nm lipoplexes at N/P = 5 ratio. In vitro transfection of the pCMV-Luciferase plasmid resulted in gene expression (>10(10) RLU/mg protein) at the same ratio, comparable with the commercially available JetSi-ENDO gene delivery system. This new and versatile class of molecules could lead to a new generation of in vivo gene delivery vectors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Polymers
/
DNA
/
Transfection
/
Cations
/
Polyynes
/
Micelles
Limits:
Humans
Language:
En
Journal:
Bioconjug Chem
Journal subject:
BIOQUIMICA
Year:
2011
Document type:
Article
Affiliation country:
France