Numerical analysis of SDS-PAGE protein patterns of Serratia marcescens: a comparison with other typing methods.
Epidemiol Infect
; 105(1): 107-17, 1990 Aug.
Article
in En
| MEDLINE
| ID: mdl-2200696
ABSTRACT
Twenty-five cultures comprising 18 clinical isolates of Serratia marcescens from two hospitals, the type strain of S. marcescens, two reference strains of S. marinorubra, the type or a reference strain of three other Serratia species and a reference strain of undetermined species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into eight protein types. Comparison with O-serotyping indicated that the level of discrimination by SDS-PAGE was similar. As with O-serotyping, a secondary scheme, such as phage typing, is necessary to differentiate strains of the same protein type. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of S. marcescens.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serratia marcescens
/
Bacterial Proteins
Limits:
Humans
Language:
En
Journal:
Epidemiol Infect
Journal subject:
DOENCAS TRANSMISSIVEIS
/
EPIDEMIOLOGIA
Year:
1990
Document type:
Article
Affiliation country:
United kingdom