Impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes.
Reprod Biomed Online
; 23(6): 704-10, 2011 Dec.
Article
in En
| MEDLINE
| ID: mdl-22019617
A prospective study was performed to assess the impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes. The study population included 178 couples (62 cycles of IVF, 116 of intracytoplasmic sperm injection (ICSI)) with own (n=77) and donor (n=101) oocytes. DNA fragmentation was evaluated by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Correlation between DNA damage to oocyte fertilization, embryo quality and clinical pregnancy, implantation and miscarriage rates was evaluated. DNA fragmentation was not related to fertilization rates in either IVF (r=0.08) or ICSI (r=-0.04) cycles. DNA fragmentation was similar in patients with <50% embryo utilization rate compared with ≥50%, in cancelled and in embryo transfer cycles and in miscarriages and in successful deliveries. Moreover, DNA fragmentation was similar in pregnant and non-pregnant women as well as in IVF with own or donor oocytes. In the multivariable analysis, the odds ratio of DNA after controlling by age was 1.0. Using a 36% sperm fragmentation threshold, results did not vary. It is concluded that DNA damage was not related to outcomes of IVF or ICSI with own or donor oocytes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Pregnancy Outcome
/
Fertilization in Vitro
/
DNA Fragmentation
Type of study:
Observational_studies
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
En
Journal:
Reprod Biomed Online
Journal subject:
MEDICINA REPRODUTIVA
Year:
2011
Document type:
Article
Affiliation country:
Spain
Country of publication:
Netherlands